Opti-Mizers

Opti-Well™

Opti-Well™ (30ml)

Opti-Well™ (30ml)

$79.00

Opti-Well™ is a rapidly curing liquid coverslip reagent that  preserves samples and fluorescent markers, eliminates meniscus distortion, and creates a flat, optically pure surface comparable to a glass coverslip. Easily adaptable to automated liquid dispensers. This enables extended storage, brighter fluorescence, and edge-to-edge phase-contrast imaging for label-free detection of most cellular features in high content imaging applications.

Opti-Well 'Auto" Liquid Coverslip for Microplates 20 mls

Opti-Well ‘Auto” Liquid Coverslip for Microplates 20 mls

$99.00

Rapidly curing storage medium with calibration beads.

  • Economical at ~3 to 7 cents per well ($79 per 30 mL)
  • Cures to refractive index of 1.5 in minutes
  • Preserves cells and fluors
  • Creates brighter, crisper images with improved signal to noise.
  • Allows edge to edge phase contrast imaging and thus labeless detection
  • Allows Label-less detection of multiple cellular features
  • Allows plate storage at -20°C for extended times
  • Allows plate shipping
  • Allows plate inversion for viewing on upright scopes
  • Optional 2.5 to 4.5 micron fluorescent beads for rapid autofocus and intensity calibration.
96 well reservoirs with no soln on top, 100 ul of PBS showing a meniscus and in the bottom well cured Opti-Well.
Empty well of a 96 well microplate, middle with 100ul PBS showing meniscal lens, bottom shows cured Opti-Well with no light aberration.
384 glass bottom Multiwell Well Plate from greiner with 7ul of Opti-well
Microspheres in one focal plane on bottom of well.
Even distribution of material in brightfield
Approx 10 microspheres per 10 objective field of view. Edge of coverslip for orientation.

Phase contrast expanded viewing area from 2% to 95% of well bottom. Revealing label-less detection of filapodia, cell area, cell membranes, growth cones, nuclei, vacuoles, stress fibers, and nucleoli.